Konstantin Gottschalk

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Effect of porosity on antibiotics encapsulation and release of melt electrospun mPCL scaffolds

 

Investigator: Konstantin Gottschalk (Master Student) Konstantin Gottschalk

 

Supervisor: Dr. Asha Mathew, Prof. Dietmar W. Hutmacher (Queensland University of Technology, Australien), Prof. Aldo R. Boccaccini

The Hutmacher laboratory and the Dalton group have demonstrated that melt electrospinning writing (MEW) is a 3D printing technique that allows the fabrication of scaffolds with controllable pore size in the micrometre range. Contrary to conventional solution electrospinning, no toxic solvents are used. This makes the scaffolds more accessible for medical applications. [1-4] In this master thesis project, medical grade polycaprolactone (mPCL) is used as the printing material, as it is known for its suitable properties for long-term drug release systems. The MEW process for the fabrication of defined pore sizes is optimized. As the main objective of this thesis is the study of the uptake and release profile of the antibiotic azithromycin in dependence of the scaffolds’ geometry, samples with different pore sizes and layer thicknesses are printed and coated with azithromycin. In addition to that, the effect of calcium phosphate coating is examined along with cell cytotoxicity.

[1] T.D. Brown, P.D. Dalton, and D.W. Hutmacher, “Direct writing by way of melt electrospinning”, Advanced Materials, vol. 23, no. 47, pp. 5651-5657, 2011.

[2] D.W. Hutmacher and P.D. Dalton, “Melt electrospinning”, Chemistry- An Asian Journal, vol. 6, no. 1, pp. 44-56, 2011

[3] T.D. Brown, A. Slotosch, L. Thibaudeau, A. Taubenberger, D. Loessner, C.Vaquette, P.D. Dalton, and D.W. Hutmacher, ”Design and fabrication of tubular scaffolds via direct writing in a melt electrospinning mode”, Biointerphases, vol. 7, no. 1-4, pp. 1-16, 2012

[4] P.D. Dalton, M.L. Muerza-Cascante, and D.W. Hutmacher, “Design and fabrication of scaffolds via melt electrospinning for applications in tissue engineering”, in Electrospinning, 2015, pp. 100-120

  

 

Fig. 1: Electrospun mPCL scaffold with a pore size of 200 μm and layerthickness of 10.

 

 

 

 

 

 

Fig. 2: Electrospun, azithromycin coated mPCL saffold with a pore size of 200 μm and a layer thickness of 20.