Thomas Distler

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Vessel bioplotting for regenerative approaches

 

Investigator: Thomas Distler (Master Student) Thomas Distler

 

Betreuer: Dr.-Ing. R. Detsch, Prof. Dr.-Ing. habil. Aldo R. Boccaccini


More than 40.000 bypass surgeries are performed yearly in Germany [1], whereas even higher numbers were estimated by the National Hospital Discharge Survey (NHDS) in the USA, e.g. 397.000 coronary artery bypass procedures in 2010 [2]. Besides the need to improve bypass constructs, also the vascularization of complex 3D scaffolds is a major challenge in Tissue Engineering. To tackle both needs, vessel like constructs have been produced in a one-step biofabrication process using a 3D BioScaffolder [3]. The method should be optimized for specific applications. In this project, by using partially-oxidized alginate (alginate di-aldehyde, ADA [4]) in combination with different proteins, a material system will be developed to fulfill the requirements of (a) being a 3D processable bioink and (b) providing optimal conditions for vascular cell types (NHDF, SMC, HUVEC).

[1] A. Beckmann, et al. Cardiac Surgery in Germany during 2012: A Report on Behalf of the German Society for Thoracic and Cardiovascular Surgery. Thorac cardiovasc Surg: 62(01): 005-017 (2014).

[2] D. Mozaffarian, et al. Heart disease and stroke statistics-2015 update: a report from the american heart association. Circulation, 131(4): e29-e322 (2015).

[3] F. Ruther, R. Singh, T. Zehnde, I. Cicha, A. R. Boccaccini, R. Detsch, Development of a biofabrication process for the manufacture of vessel like structures. BioNanoMaterials. 17, 181 (2016).

[4] B. Sarker, et al., Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties. Journal of Materials Chemistry B 2, 1470-1482 (2014).