Antibacterial chitosan-based composites for tissue engineering

 

Investigator: Yeseul Sim (Master Studentin) Yeseul Sim

 

Betreuer: Lukas Gritsch, Prof. Aldo R. Boccaccini

Chitosan is a biopolymer obtained from the alkaline deacetylation of chitin, a main component of the outer skeleton of shrimps, crabs and insects. Chitosan has been known as an antimicrobial material against bacteria and fungi[1,2]. Additionally, the reactive functional groups present in polymer chains (e.g. amino group and hydroxyl group) increase the biocompatibility of chitosan since they readily allow adjustment of mechanical and solubility properties [2]. Therefore, chitosan, in its various forms, has been investigated as a potential candidate for tissue engineering applications. For example, chitosan films have been tested as curative wound healing dressing and as scaffolds for bone regeneration [1]. In this project, chitosan-based films and porous scaffolds will be respectively fabricated by solvent casting and solvent casting/particulate leaching methods. Meanwhile, selected amounts of bioactive glasses in particulate form will be used to improve the strength and bioactivity of chitosan films and scaffolds exploiting the composites approach [3]. Characterization of obtained chitosan-based composites in terms of morphological, biodegradable, and structural studies will be performed. Furthermore, the antibacterial activity and cytocompatibility of developed chitosan-based composites will be investigated.

[1] EI Rabea et al., Chitosan as Antimicrobial Agent:  Applications and Mode of Action, Biomacromolecules 4(6): 1457–1465 (2003)

[2] Rejane C. Goy et al., A Review of the Antimicrobial Activity of Chitosan, Polimeros 19(3):241-247 (2009)

[3] Boccaccini A.R., Erol M., Stark W.J., Mohn D., Hong Z., Mano J.F., Polymer/bioactive glass nanocomposites for biomedical applications: a review. Composites Science and Technology 70: 1764–76 (2010)